learn about titrations yet, that's fully fine. pH: 5.5 0.15 Storage: Room Temp. pKa (25C) pKa (37C) cat.number MES buffer pH 5.2-7.1 14035 213.2(h) pKa=6.16 6.15 5.97 Bis-Tris buffer 209.2 pH 5.8-7.2 36832 -6.5 6.36 . Below are tables that include determined pKa values for various acids as determined in water, DMSO and in the gas Phase. pH: 6.0 0.15 Storage: 2-8C, Thomas Scientific 2023 All Rights Reserved. MES does not form radical species, making it suitable for redox studies 5. K_a is just equal to H_three O plus times A minus all over the concentration of HA, and we don't include water Lah, MS; Palfey, BA; Schreuder, HA; Ludwig, ML. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH. Add the following amounts of 0.2 M HCl per 100 ml cacodylate stock solution, followed by the addition of DI to a final volume of 400 ml, to obtain 0.05 M cacodylate buffer at the desired pH (Dawes, 1971). So let's go ahead and look at all the possible scenarios for these three things. So anything to the zeroth Plant Microtechnique and Microscopy, Use TBS when performing immunocytochemical, experiments on phosphate-sensitive tissues, Tris base DI Dissolve and adjust pH with the following approximate amount of HCl: pH 7.4 pH 7.6 pH 8.0, Disodium ethylene diamine tetraacetate Adjust pH to approx. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 - 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. ,d6R J%:LN*0B1'@PP">& "7/ubjM~O~O~OXTGl5-}YL+hdyd24a. Impurities:Endotoxin 50 EU/gYeast and Mold 100 CFU/gBioburden 100 CFU/gAl 5.0 ppmAs 3.0 ppmBa 5.0 ppmBi 5.0 ppmCa 20.0 ppmCd 5.0 ppmCo 5.0 ppmCr 5.0 ppmCu 5.0 ppmFe 5.0 ppmLi 5.0 ppmMg 5.0 ppmMn 5.0 ppmMo 5.0 ppmNa 20.0 ppmNi 5.0 ppmPb 5.0 ppmSb 5.0 ppmZn 5.0 ppm. Biochemistry 33, 1555- 1564, 1994. What would you add to this before you brought the volume to 1 L with water? Tris buffers are used commonly in microtechnique applications involving molecular biological procedures. It is recommended for separating small- to medium-sized proteins. Direct link to Saksham Sodani's post cant we have buffers with, Posted 7 years ago. 3FV4ZrPmLRhbiM}~^RiJc0;SMa So there are two other possibilities for pH and pK_a. MES Free Acid is a buffer used often in plant tissue culture media with a pKA = 6.1 at 25C, 2-(N-morpholino)ethanesulfonic Acid MES, Free Acid JavaScript seems to be disabled in your browser. Since this Good's Buffer lacks the capability to form a complex with most metal ions, therefore it is recommended for use as a noncoordinating buffer in solutions with metal ions. two things are related to the relative size of pH and pK_a. [2] MES is highly soluble in water. All rights reserved. <> To 50 ml of 0.2 M sodium barbital (Veronal. MES is used as a buffering agent in biology and biochemistry. Keep buffer concentration as low as possible yet enough to maintain pH. Posted 7 years ago. It's very easy to derive. And the point in your titration where the HA is equal to A minus is called the half-equivalence point. Titration 1. Solutions are stable at 2-8C for months. MES was developed as one of 'Good' stamps in the 1960s. MOPS, 3-(N-Morpholino)Propanesulfonic Acid, MES, 2-Morpholinoethanesulfonic acid, are commonly used in biological experiments, and played very important role. MES Buffer is a zwitterionic buffer and is primarily used as a running buffer for resolving very small proteins on bis-tris gels. MES Buffer is a remarkably chemically and enzymatically stable zwitterionic buffer. We're going to start with the simplest possible scenario, which is that pH is equal to pK_a. Crystallization Plates, Hardware & Accessories, Crystallization grade MES monohydrate for formulating screens or for optimization, Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/L). HR2-943-07 - MES monohydrate pH 5.8 - 1.0 M solution is a Custom Shop reagent and is used to reproduce and optimize Crystallization Reagents for use with the Silver Bullets kit. Prepare a 0.2 M stock solution of sodium cacodylate in water (4.28 g/100 ml). is actually greater than the denominator, HA. MES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. Direct link to Amanda Saideepane's post If exactly half the amoun, Posted 2 years ago. x}"tOUFC],yyTLk{'reV&@R\ ra!7_?>9472Mwt_vxga8g=r.X{?? derive the other relation. remember this all the time. Co., Ltd - Fine Chemicals Division. This tells us that the reaction is essentially irreversible, or it only goes in one direction; towards the products with very little reactants reformed in the reverse direction. [ 5] Other Notes No, as the 10 is added due to the log of [A-]/[HA] which is positive. : 117961-21-4 Reconstitution: Contents of each pouch dissolved in 1 liter deionized, PAGEmark Blue PLUS Protein Markers A blue protein standard with 12 prestained proteins covering a wide range of molecular weights:10-240kDa in Tris-Glycine buffer and9-235kDa in Bis-Tris (MOPS) buffer or Bis-Tris (MES) buffer. If you are sure about your decision, please writedown your e-mail and click onunsubscribe. Direct link to Jesi Buckley's post At 3:55, shouldn't the pH, Posted 4 years ago. So we're gonna subtract pK_a from both sides and that gives us a log of A minus over HA. Analytical tests (DNase, RNase, protease activity, heavy metals and others upon request), 15 business days after order confirmation, Product registered under the European Chemicals Agency's REACH regulation, ADD: No.28, Huadong Road, Daliao District, Kaohsiung City, 831, Taiwan. When pH is equal to pK_a, we're raising 10 to the zeroth power. The pH (and pKa at ionic strength I0) of the buffer solution changes with concentration and temperature, and this effect may be predicted using online calculators. This is, as written, it's the acid dissociation Since this Good's Buffer lacks the capability to form a complex with most metal ions, therefore it is recommended for use as a noncoordinating buffer in solutions with metal ions. what happens to the buffer ratio if the pH is raised? deprotonated). MES is a popular choice for buffered culture media for bacteria, yeast and mammalian cells. C(]]`/\pctf.j87 =qRKO5M-*Ou&\oXMNTQ}bY!8#)Z,y^\`'c3Dw~WcWMX]O r Lq$Hcp 1 0 obj Its chemical structure contains a morpholine ring. MES is not a good buffer for pH 4 because the pKa of MES is 6.1. v6P0\?@g%]?32:Q-(^:o"ch[[v9 MQ|uX}n; ~yk?flo5g`[kckKk6;OS?rk@/{Bw2v,LY`+3\,;a, S2u}V7;KCn*t--XeUJ3w5t`z[k+ o-2Iug}s#1{^wKX]O?rZ&U|epWf|LSj)}M6qSKmJ`87nw[).%\Sn8ujwaU9(O{`@vHAw]?5i:4IiovpKk%Ex rR_z+ X pH and pKa relationship for buffers (video) | Khan Academy kinds of information. name, 'cause it happens to be for the dissociation of an acid. So the pH of our buffer solution is equal to 9.25 plus the log of the concentration of A minus, our base. ta 2aur;V.H'LuT?b>x*:7"Y%sujq.oKLD s sJs+H+HpPA7t'jaVrO7$^m|g6q48Ow*1Y\x Any time you have a buffer and the pH of your solution is equal to the pK_a of your buffer, you immediately know Thermo Fisher Scientific. Not for use in diagnostic procedures. MOPS ( 3- (N-morpholino)propanesulfonic acid) is a buffer introduced in the 1960s, one of the twenty Good's buffers. MES monohydrate Buffer - Hampton Research In the aqueous buffer solution, H3O+, HA and H+ exist in equilibrium with each other. ;|c]?^~OorOO7/}_}w{/8|f>k{?`8tmz}v=]>F/@-~OyH?h`/0cW`{7r\Nq}8v/?Gy;}cOW.7=~4\\Gh0N =oOaw7{_u~dx{gq9ebl,\c~|wh{~;a?P{gru3]0}28|l~M@w~%vr.D?c_{t&4e< #P;MGpTo@Hkc?}2r6`vB_?^yhq/q,{px76? derive a couple relationships. MES, Free Acid, Ultrol Grade - CAS 4432-31-9 - Calbiochem A When pH is equal to pK_a, we're raising 10 to the zeroth power. HEPES is a similar pH buffering compound that contains a piperazine ring. Need suggestion if 1:10 dilution of the MES buffer (0.5 Molar; to 0.05 Molar), with pure water (MQ, pH around 5.6), would have impact. p Ka: Because most biological reactions take place near-neutral pH between 6 and 8, ideal buffers would have pKa values in this region to provide maximum buffering capacity there. MOPS | CAS 1132-61-2 - - Hopax Fine Chemicals TrisHCl (pKa = 8.06) and maleate (pKa = 6.26) have a working range of pH 5.08.6 and may be used successfully to buffer staining solutions (. MES is a zwitterionic buffer. (4) Make the volume to 1L, then filter with 0.45m filter membrane to remove impurities, store at room temperature and protect from light. Henderson-Hasselbalch equation, which I am gonna assume Is going to give us a pKa value of 9.25 when we round. Change in pH of MES buffer with dilution in pure water? Further reading: How to choosing the biological buffer you need: By ph and pKa. If you alter that the equation, by removing the p as well, then it gets kind of complex to look at but it can be done. . It is not absorbed through cell membranes, and it is essentially transparent to UV light. MES (buffer) - Wikipedia What do you call a reaction where the pH of two substances is brought closer to 7? Presented here are three common formulations (Mishkind, Sodium cacodylate buffer [Na(CH3)2 AsO2 3H2O] is a alternative to Srensens phosphate buffer. So if we are throwing them into water, the Hydrogen Ions begin to mix making more H2O(l) molecules. 3 0 obj Text . Synonyms include: 2-morpholinoethanesulfonic acid; 2-(4-morpholino)ethanesulfonic acid; 2-(N-morpholino)ethanesulfonic acid; 2-(4-morpholino)ethanesulfonic acid; MES; MES hydrate; and morpholine-4-ethanesulfonic acid hydrate. not super-intuitive sometimes. PDF Useful pH Ranges of Selected Biological Buffers (25 C, 0.1 M) And I said really a lot there. Just ignore what I just said (laughs), but if you have, the moral is just that, this is a really, really Solved Question 12 1 pts You are making some MES buffer. The - Chegg Identify which Good's buffers are most effective near pH 7.0 and near pH 6.0. 5 ml of 10 M HCI 50 mL of 10 M HCI O 5 ml af 10 M NaOH 50 mL Show transcribed image text Optimize Reagents I feel that you're correct. contains both a weak acid, which generically we write as HA, and it also contains in equilibrium the conjugate base of If you're seeing this message, it means we're having trouble loading external resources on our website. %PDF-1.7 The log in pH is left untouched. Mix the three purified enzyme components of NBDO in 50 mM MES buffer at pH 6.8 containing 100 M (NH 4) 2 Fe(SO 4) 2 to final concentrations of 0.3 M reductase, 3.6 M ferredoxin, and 0.3 M oxygenase.. 2. MES was developed as one of Good's buffers in the 1960s. to get rid of the log by raising both sides to the 10th power. It has the advantages of: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pK with temperature chemically and the same concentration. to solve for this ratio that we might be interested in. Ut4SL@PIZ=E L^Q,Z rYd`.Hald'}G".$dX%Rl"LZ2Jr% x So if we look at this, we can (5) Complex with organotin (IV) molecules as antitumor agents. High concentrations of MES are toxic to most plants, but can be used in plant media at concentrations ~10 mM; (4) Electrophoresis and chromatography including capillary electrochromatography, gel filtration chromatography, phosphocellulose column chromatography, hydrophobic interaction chromatography, cation exchange chromatography and SDS-PAGE; Assume that the pH is 6 and the concentration is 100mM: (1) 0.1mol or 19.5g of MES was weighed and dissolved in 500mL of DEPC water; (2) The pH was adjusted to 6.0 with 1N or 10N NaOH solution; Application of Tris|CAS:77-86-1| in the field of coatings, Application of guanidine hydrochloride|CAS:50-01-1| in the treatment of cancer/tumor, Application of Tris|CAS:77-86-1| in eye drops, Application of Guanidine Hydrochloride|CAS:50-01-1| in Energy and Chemical Industry, Application of guanidine hydrochloride|CAS:50-01-1| in the field of graphene preparation, Guanidine HCL Guanidine hydrochloride CAS 50-01-1, 99.5% purity HEPES CAS:7365-45-9 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid, N-Cyclohexyl-3-aminopropanesulfonic acid CAS1135-40-6. Terms & Conditions, By submitting your email address, you are agreeing to our, MES hydrate free-flowing, Redi-Dri(TM), >=99.0%. This article is about the biochemical buffering agent. HEPES Sodium is considered to be non-toxic to culture cell . Change in pH of MES buffer with dilution in pure water? Henderson-Hasselbalch equation to get a lot of different simplest possible scenario, which is that pH is equal to pK_a. Solved Good's buffers are compounds that are useful near - Chegg And if A minus concentration over HA concentration is equal to one, that means that they have > MES monohydrate Buffer, Synonyms: MES or 2-(N-morpholino)ethanesulfonic acid or 4-morpholineethanesulfonic acid monohydrateC6H13NO4S H2OMr 213.25CAS Number [145224-94-8]EC Number 224-632-3Beilstein Registry Number 329824236RTECS QE3479500MDL Number MFCD00149409Purity 99.5%pKa 6.1 at 25C. The buffering mechanism consists of two reversible reactions where the . So if our ratio A minus over HA is greater than one, that tells us that A minus, the numerator, A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =1). Its chemical structure contains a morpholine ring, MES buffer has a buffer range of pH 5.8 to 6.5. Mes-Buffer at Thomas Scientific Expert Answer. This is why we excluded H2O (L) in the ICE tables as well. Used in different types of chromatography, such as gel-filtration, phosphocellulose column, hydrophobic interaction and cation exchange chromatography. Identification and Characterisation of a pH-stable GFP The pKa is equal to the pH in that scenario, Buffers, titrations, and solubility equilibria. endobj ~k {,j3,vY"ZX kH|{/rOr8 sX+G(ux~z}7mH;#G{K=5W?77|wkk8\dn^54n?7y6q7?S/O>`}@Lgwvfy~_Q8K|7IRn_Yn,lo!7|w-t_P[?9Y|n^xB.WFG^;=^is2]%7Nzou uI#vl+cq};HslxG#qS8 eJyQ}EpVd S;Zufhx4Fq?legnL{bEkLC0ZKZY/#y!_MY\)k{?lW10SA:tW_9c_\z]kj/M7#5[@vRwwe}F'Ke\XnqW}C6~uw%w\ KTpQdpO7cug?WOvn_[.lUWRcTXQdokka15ot_a.8o][2Q^Iyr'5~@0J,vow c\/}R}e(F8,oSS\f.?BNdp.]cq'}LBbo!dbo8l=V/B;EvU5K{|Ruk0Mosl{_js6~x}6v;0c9n=I.;Z_?+0h_:GZ")o,{`\pq] Plant Microtechnique and Microscopy, CACODYLATE BUFFER; PH 5.07.4, PKA = 6.27. We did it in just a few minutes, so it's okay if you don't Buffer Table Crystal Growth 101 page 1 Buffer . let's do a quick review of what exactly is a buffer. Compound name: MES buffer. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). Click on Continue. a pH of ~ 6.1. The temperature dependence of a buffer system is expressed as d(pKa)/dT, which describes the change of the pK a at an increase of temperature by 1C. MES, Free Acid | GoldBio Direct link to Khan academy's post No, as the 10 is added du, Posted 6 years ago. endobj The useful buffer range for tris (pH 7-9) coincides with the physiological pH typical of most living organisms. Use our interactive chart below to compare the useful pH range of this product with other buffers. constant for this equation. other videos introducing it and also deriving it, I usually just remember For a buffer to be most effective . Buffers - The Biological Imaging Facility - University of California What is the protocol of MES buffer preparation? | ResearchGate Copyright Suzhou Yacoo Science Co., Ltd. All Rights Reservedvar cnzz_protocol = (("https:" == document.location.protocol) ? " Direct link to Jonathan Acosta Hernandez's post We consider H2O(l) as 1 b, Posted a year ago. Direct link to Ernest Zinck's post Chemical equation: B + H, Posted 7 years ago. If the product you are interestedis available only in bulk quantities, click on "Request a Quotation". The last scenario, pH is less than pK_a, well in that case, we're raising 10 to a negative number, because we're subtracting a bigger SODIUM ACETATE; PH 3.6-5.6, PKA = 4.76 We can have a pH that's greater than pK_a for your buffer, and you can have a pH that is less than you In addition, the experimenter should pay attention to the sterilization of containers, water and other additives. *. conjugate base are the same. This is a really helpful Buffers work better within 1 unit of the pKa. <>/Metadata 1837 0 R/ViewerPreferences 1838 0 R>> [2] MES is highly soluble in water. It has good pH buffering capacity within the range of pH 5.07.4. <> to a positive number, when you raise 10 to a positive number, you get a ratio that is greater than one. Oh, sorry, if you haven't After confirming the order, you will receive an email with your invoice and the instructions to make your payment. So if your pH is bigger than your pK_a, then this term up here, 10 to the pH minus pK_a, So a buffer is something that contains an aqueous solution. Synonym (s): 2- (N-Morpholino)ethanesulfonic Acid Empirical Formula (Hill Notation): C6H13NO4S CAS Number: 4432-31-9 Molecular Weight: 195.24 MDL number: MFCD00006181 And as you can see up here, an acid and its conjugate base are just related by the fact These buffers were developed with the following criteria in mind: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and reasonably easily synthesized. PDF User Guide for GoldBio Buffers - user560 Apr 6, 2012 at 4:10 @leonardo. It tells us about the PDF MES Buffer 0.5M Stock Solution with table - goldbio.com Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Spectroscopy, Elemental and Isotope Analysis, Protein Electrophoresis & Western Blotting, Compare protein migration patterns using MES and MOPs on NuPAGE Bis-Tris gels, See all available buffers and reagents available for SDS-PAGE. Design by 37design. MES (Buffer) - an overview | ScienceDirect Topics Henderson-Hasselbalch equation. Direct link to Khan academy's post The Buffer ratio increase, Posted 3 years ago. actually less than one. Tris - Wikipedia Hopax is a manufacturer and supplier of MES (CAS 4432-31-9), a zwitterionic biological buffer often used as a buffering agent in biological and biochemical research. So that tells us that our denominator, HA, is actually bigger than our numerator. 1PF&P@nMQDDv$ But before we go to the A biological buffer mainly used in plant cell cultures. So what we're gonna do, is we're gonna rearrange this equation Further reading: Useful pH range of Biological Buffers. Direct link to Ryan W's post The pKa is equal to the p. Mes 145224-94-8 you've seen before, and if not we have some MES only weakly binds Ca, Mg, Mn, and it has negligible binding with Cu(II). In personal care and cosmetics applications, MES can protect the active ingredients of the formulations against degradation. ratio is equal to one. When MES solutions are stored at room temperature, they can turn yellow within six months. Direct link to s.m.guillaume's post at 1:32, correct me if I', Posted 7 years ago. MOPS is a similar pH buffering compound which contains a propanesulfonic moiety instead of an ethanesulfonic one. Assay (titr. Used for mammalian cell culture 8. Add the following to create 200 ml of buffered solution. You must choose a buffer that has a pKa value near the middle of the range required (in general, it is recommended to use a buffer with a pKa value that is at least within one pH unit of the target pH). One of the original Good's buffers, MES Buffer has a pKa value near physiological pH. Solution: The Henderson-Hasselbalch equation, which links the pH of a buffer solution to its acid dissociation constant (pKa) and the ratio of the concentrations of the conjugate base (A-) and acid (HA) forms of the buffer, must be used to calculate the pH of a 0.35 M MES buffer. Language links are at the top of the page across from the title. please read on, stay posted, subscribe, and we welcome you to tell us what you think. Not Intended for Diagnostic or Therapeutic Use. It has pKa value of 6.15 at 20C. (3) Make the volume to 1L, preserve at 4C. [3] MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.[4]. A buffer consists of a weak acid (proton donor, HA) and its conjugate base (proton acceptor, A -). Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA). Application: MES, Sodium Salt is a buffering agent used in biological and biochemical research including plant cell culture CAS Number: 71119-23-8 Purity: 99% Molecular Weight: 217.22 Molecular Formula: C 6 H 12 NO 4 SNa For Research Use Only. So we're gonna plug that into our Henderson-Hasselbalch equation right here. Can be used for either transient or stable transfection procedures. Not all buffers will be used. Which tells us that this ratio is equal to one. on reaction for HA and since it's an equilibrium we can write an expression called K_a, which is just the equilibrium Mix appropriate volumes of stock and add an equal volume of distilled water to make a final 0.1 M Srensens phosphate buffer solution (Srensen, 1909; Gomori, 1955). So pKa is equal to 9.25. MES Buffer MOPS; ; RNA; (Lysis Buffer)(Running Buffer); . Also used in c Used for in vitro dissolution testing of liposomes 10. MES buffer - Microbiologie clinique If you still have any questions, feel free to contact us at. You wanna know what's going on. Bordwell pKa Table - Organic Chemistry Data CITRATE BUFFER; PH 3.06.2, PKA = 6.40 Citrate buffer (Gomori, 1955) stock solutions: A: 0.1 M citric acid; B: 0.1 M sodium citrate. [2] Usage above 20 mM in mammalian cell culture work is not recommended. Autoclaving is not recommended for any sulfonic acid buffer. For example, a buffer with a pH of 6.8 has a pH buffering range of 5.8-7.8. you, but I actually find, well, (laughs) I find logs Applications and Preparation of MOPS and MES Buffer - Yacooscience ,PLPPAc26LF)aw0\-3-[s"2H1AL1O1 H1OJ1OJ1)L1M1f_O|9_8b_\XEVyqlh^DmEHs^V%xv!"'-(Bh"osD3@A @BY!6L#DB:k8h(gq~"dZuy]?F=S To make a 1 L solution of 0.5M MES, use the table below to estimate the required volume of base for a given pH: Starting pH: 3.23 Adjust pH with: 10N NaOH Why are all these eqaution dealing with weak acids, why doesn't it work for strong acids?! >g}pxyW!Wwo_6W/ Language links are at the top of the page across from the title. From Plant & Animal Microtechnique, SE Ruzin, Oxford Univ Press, 1999, From Ruzin, 1999. HEPES Sodium, used in conjunction with HEPES, is the most commonly used biological buffer formulation since the pKa of the buffer is 7.31 which corresponds to the physiological pH of 7.36. MOPS - Wikipedia MES is used as a buffering agent in biology and biochemistry. Keep in mind that high levels of phosphate may be somewhat toxic to plant cells (Sabatini, Add the following to create 100 ml of phosphate/citrate buffer solution. Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. 300C. How to choose the right biological buffer - Hopax Fine Chemicals 4 0 obj The pH of the buffer indirectly affects this because it will determine the net ion balance based on the pKa values of MES/MOPS, so you will get different results using the same buffers but at different pH values. The pka of MES is 6.2. Components: MES Monohydrate(500 mM), Sodium Hydroxide(for pH adjustment), Water(Rest) Method: Prepared in 18.2 megohms-cm 1 water and filtered through 0.22-micron filter.
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